|
| Status |
Public on Jun 24, 2011 |
| Title |
Macrophage control #1 treated with medium only |
| Sample type |
RNA |
| |
|
| Source name |
Murine J774.A1 macrophages, no infection
|
| Organism |
Mus musculus |
| Characteristics |
treatment: no infection
cell line: Murine J774.A1 macrophages
|
| Treatment protocol |
No specific treatment. This is a control for B. suis strain S1330-infected macrophage study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Total RNA (20 µg) per sample was used for hybridization with Affymetrix mouse GeneChip 430 2.0 array at the Core Laboratory Facility at the Virginia Bioinformatics Institute (VBI).
|
| Scan protocol |
Follow standard Affymetrix protocol.
|
| Description |
This is a control macrophage sample treated with only medium (no bacterial infection).
|
| Data processing |
The data was processed using RMA.
|
| |
|
| Submission date |
Mar 29, 2010 |
| Last update date |
Jun 24, 2011 |
| Contact name |
Yongqun He |
| E-mail(s) |
[email protected]
|
| Phone |
734-615-8231
|
| Fax |
734-936-3235
|
| URL |
http://www.hegroup.org/
|
| Organization name |
University of Michigan
|
| Street address |
018 ARF, 1150 W. Medical Center Dr.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21117 |
Microarrays of macrophages infected with smooth or rough Brucella suis |
|
