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| Status |
Public on May 01, 2021 |
| Title |
J_R_0h_rep1 |
| Sample type |
SRA |
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| Source name |
root
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| Organism |
Glycine max |
| Characteristics |
tissue: root
genotype: Wild type
treatment: untreated
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| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent method (Invitrogen)
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
JACK, untreated
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| Data processing |
RNA-sequencing was performed using an Illumina instrument of NovaSeq 6000
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Glycine max whole genome using Hisat2
Reads counting was performed using featureCounts software.
Differential expressed genes were analyzed using DESeq2 software.
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| Submission date |
Apr 30, 2021 |
| Last update date |
May 02, 2021 |
| Contact name |
Wei Wei |
| E-mail(s) |
[email protected]
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| Phone |
8610-64807602
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| Organization name |
Institute of Genetics and Developmental Biology
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| Street address |
Chaoyang District, Beichen West Road, Campus #1, No 2
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platform ID |
GPL28801 |
| Series (1) |
| GSE173640 |
Next Generation Sequencing Quantitative Analysis of soybean seeds Transcriptomes |
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| Relations |
| BioSample |
SAMN18929622 |
| SRA |
SRX10722218 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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