|
Status |
Public on Apr 01, 2022 |
Title |
KO 1 |
Sample type |
SRA |
|
|
Source name |
Liver endothelium
|
Organism |
Mus musculus |
Characteristics |
tissue: Liver genotype: c-Maf KO
|
Extracted molecule |
polyA RNA |
Extraction protocol |
ECs were isolated by fluorescence-activated cell sorting and subjected to RNA extraction using RNeasy Mini Kit from Qiagen. RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
MAF_KO_MCR_148_S57_L005_R1_001
|
Data processing |
FastQC (v0.11.5) to check FASTQ quality. Skewer (v0.2.2) for adapter trimming Minion from Kraken tool (reaper-13-100) for adapter diagnosis STAR (v2.7.6a) for alignment and gene count quantification using --quantMode GeneCounts Genome_build: GRCm38 Supplementary_files_format_and_content: Gene count matrix
|
|
|
Submission date |
Apr 19, 2021 |
Last update date |
Apr 01, 2022 |
Contact name |
Jesus Maria Gomez Salinero |
E-mail(s) |
[email protected]
|
Organization name |
Weill Cornell Medicine
|
Street address |
1300 York Avenue
|
City |
NEW YORK |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL21103 |
Series (2) |
GSE172360 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization [mouse liver] |
GSE172362 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization |
|
Relations |
BioSample |
SAMN18797635 |
SRA |
SRX10637048 |