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Sample GSM5253191 Query DataSets for GSM5253191
Status Public on Apr 01, 2022
Title KO 1
Sample type SRA
 
Source name Liver endothelium
Organism Mus musculus
Characteristics tissue: Liver
genotype: c-Maf KO
Extracted molecule polyA RNA
Extraction protocol ECs were isolated by fluorescence-activated cell sorting and subjected to RNA extraction using RNeasy Mini Kit from Qiagen.
RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description MAF_KO_MCR_148_S57_L005_R1_001
Data processing FastQC (v0.11.5) to check FASTQ quality.
Skewer (v0.2.2) for adapter trimming
Minion from Kraken tool (reaper-13-100) for adapter diagnosis
STAR (v2.7.6a) for alignment and gene count quantification using --quantMode GeneCounts
Genome_build: GRCm38
Supplementary_files_format_and_content: Gene count matrix
 
Submission date Apr 19, 2021
Last update date Apr 01, 2022
Contact name Jesus Maria Gomez Salinero
E-mail(s) [email protected]
Organization name Weill Cornell Medicine
Street address 1300 York Avenue
City NEW YORK
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL21103
Series (2)
GSE172360 Temporal and functional determinants of intra-hepatic vascular homeostatic specialization [mouse liver]
GSE172362 Temporal and functional determinants of intra-hepatic vascular homeostatic specialization
Relations
BioSample SAMN18797635
SRA SRX10637048

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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