|
| Status |
Public on Jul 19, 2010 |
| Title |
ascending colon biopsy from healthy subject (GSM282892) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ascending colon biopsy from healthy subject
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 105
current medication: None
birth date: 7/29/84
ethnicity: CAUCASIAN
symptoms onset date:
diagnosis date:
ocp:
joint problems: FALSE
family history: FALSE
aza treated:
aza tolerant: FALSE
procedure date: 11/15/04
indictation for procedure: IBS type symptoms
cdai:
harvey bradshaw:
esr:
crp:
hb:
wcc:
neutrophils:
albumin:
blood obtained: FALSE
ibd affected relatives:
igr2096:
igr2198:
igr2230:
octn1:
octn2:
nod 702:
nod 908:
dlg5 133a:
nod 1007 fs:
further operations:
stoma ileal: FALSE
diagnosis oral: FALSE
diagnosis ileal: FALSE
diagnosis colonic: FALSE
diagnosis rectal: FALSE
diagnosis anal perianal: FALSE
diagnosis vienna classifi:
last followup date:
followup oral: FALSE
followup ileal: FALSE
followup colonic: FALSE
followup rectal: FALSE
followup anal perianal: FALSE
followup vienna classific:
casenote review date:
behavior at diagnosis:
diagnosis stricture fistu:
diagnosis details:
behavior at followup:
disease group:
smoking status:
smoking start date:
smoking stop date:
smoking amount:
other illnesses:
disease: healthy
treatment: control
anatomic location: ascending colon
treatment response: NA
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA (Catalog #740000)
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Stratagene Universal Human Reference RNA
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not applicable, Universal Human Reference RNA (Catalog #740000) purchased from Stratagene
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| |
| Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
| Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
| Description |
ascending colon biopsy from healthy subject
|
| Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Apr 12, 2017 |
| Contact name |
Alex Abbas |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE20881 |
Colon biopsies from Crohns patients and healthy controls |
|
| Relations |
| Reanalysis of |
GSM282892 |
