|
| Status |
Public on Mar 10, 2011 |
| Title |
663LNA LS3 |
| Sample type |
RNA |
| |
|
| Source name |
663LNA LS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HUVEC
transfection protocol: miR-663 knockdown
shear stress: LS
|
| Treatment protocol |
HUVEC were transfected with control or miR-663 LNA (100uM) and then subjected to shear stress (oscillatory or laminar shear stress) for one day
|
| Growth protocol |
HUVEC were cultured and used between passage 5-6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from HUVEC using miReasy kit (QIAQEN)
|
| Label |
biotin
|
| Label protocol |
According to Illumina protocol.
|
| |
|
| Hybridization protocol |
According to Illumina protocol.
|
| Scan protocol |
According to Illumina protocol.
|
| Description |
replicate 3
|
| Data processing |
The miRNA data were quantile-normalized within Illumina GenomeStudio.
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Mar 10, 2011 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
[email protected]
|
| Phone |
404-712-9654
|
| Fax |
404-727-3330
|
| Organization name |
Emory University
|
| Department |
Biomedical Engineering
|
| Street address |
2005 WMB
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30068 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE20739 |
Gene expression analysis of miR-663 knockdown under shear stress in HUVEC |
|
