|
| Status |
Public on Feb 17, 2011 |
| Title |
Human hepatocellular carcinoma cell line infected with MIR517A and GFP protein 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human hepatocellular carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
infection: MIR517A and GFP protein
|
| Treatment protocol |
Recombinant lentivirus were produced by co-transfecting human embryonic kidney 293T cells with miR-517a or empty vector (ViraPower™ Lentiviral Packaging Mix, Invitrogen, Carlsbad, CA) using Lipofectamine 2000. Supernatant containing lentivirus was collected 48h after transfection. Huh-7 cells were transduced with the lentivirus. GFP+ cells were sorted by fluorescence-activated cell sorting (FACS) analysis (Becton Dickinson Instrument, San Jose, CA).
|
| Growth protocol |
Cells were cultured in DMEM medium with 10% FBS at 5% CO2, 37oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Biotin
|
| Label protocol |
Followed manufacturer's protocol
|
| |
|
| Hybridization protocol |
Followed manufacturer's protocol
|
| Scan protocol |
Followed manufacturer's protocol
|
| Description |
hcc04mi16
|
| Data processing |
Signal intensities were normalized using RMA algorithm implemented in GenePattern ExpressionFileCreator module (http://www.broadinstitute.org/genepattern).
|
| |
|
| Submission date |
Mar 08, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Yujin Hoshida |
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE20596 |
MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a |
| GSE20679 |
mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line |
|
| Relations |
| Reanalyzed by |
GSE86357 |
