|
| Status |
Public on May 10, 2010 |
| Title |
sip2gal83 deletion_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
sip2gal83 deletion_biological rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: sip2Δ gal83Δ
|
| Treatment protocol |
biomass samples were taken at steadystate, cooled down immediately and centrifuged with cooling. The cell pellets were frozen in liquid nitrogen.
|
| Growth protocol |
strains were grown in 1 liter bioreactor at 30°C, pH 5.0 and aerobically, 10 g/L glucose, dilution rate 0.1h-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen cell pellets using FastRNA® Pro RED kit (QBiogene, Inc, USA)
|
| Label |
Biotin
|
| Label protocol |
Labelling was done according to the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Hybridization was done according to the Affymetrix GeneChip Expression Analysis Technical Manual on a GeneChip Fluidics Station FS-400.
|
| Scan protocol |
Scanning was done according to the Affymetrix GeneChip Expression Analysis Technical Manual on a GeneChip Scanner 3000.
|
| Description |
sip2gal83 deletion_biological rep2
|
| Data processing |
Affymetrix CEL-data files were preprocessed using the statistical language and environment R Language version 2.6. The probe intensities were normalised for background using the robust multiarray average (RMA) method (Irizarry, 2003) using only perfect match (PM) probes. Normalization was performed subsequently using the qspline algorithm (Workman, 2002). Gene expression values were calculated from the PM probes with the medianpolish summary method (Irizarry, 2003). All statistical preprocessing methods were used by invoking them through the affy package (Gautier, 2004).
|
| |
|
| Submission date |
Mar 02, 2010 |
| Last update date |
May 10, 2010 |
| Contact name |
Jie Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
+45 52710377
|
| Organization name |
Technical University of Denmark
|
| Department |
CFB
|
| Lab |
SBTY
|
| Street address |
Kemitorvet 220
|
| City |
Kgs. Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE20591 |
Expression data of β-subunit of Snf1 kinase in yeast Saccharomyces cerevisiae |
|
