|
| Status |
Public on Jun 09, 2021 |
| Title |
Senescent cell, dep |
| Sample type |
RNA |
| |
|
| Source name |
biliary epithelial cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: biliary epithelial cell
treatment: serum depletion
|
| Treatment protocol |
Cellular senescence was induced in BECs cultured in vitro incubation in serum depleted media or treatment with media containing 200 μM GCDC for 4-7 days
|
| Growth protocol |
Biliary epithelial cells are cultured in DMEM/F-12 with 10%Nu-serum, EGF, insulin, forskolin and antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using RNesy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Total RNA was extracted and analyzed
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Mar 02, 2021 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22438 |
| Series (1) |
| GSE168052 |
Gene expression profiles of senescent biliary epithelial cells induced by serum depletion and GCDC |
|
