|
Status |
Public on Feb 23, 2021 |
Title |
Donor 2-CD8+CD161- alt splice |
Sample type |
RNA |
|
|
Source name |
Peripheral blood PBMC
|
Organism |
Homo sapiens |
Characteristics |
cells: CD8+CD161- T cells treatment: PDAC antigen-loaded autologous DC
|
Treatment protocol |
CD8+CD161+ and CD8+CD161- T cells were magnetically sorted from healthy donor peripheral blood.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from the cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. Inclusion of 10 mL/L of the RNase inhibitor NucleoGuard (AmpTec) in the lysis buffer improved RNA yield and recoveries of long transcripts. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
|
Label |
biotin
|
Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
|
|
Hybridization protocol |
Samples were hybridized with GeneChip Human WT Pico-Clariom D Exon Arrays (Affymetrix) and scanned at the Sequencing and Microarray Facility at the University of Texas MDAnderson Cancer Center.
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
Description |
Donor 2-CD8+CD161- RMA expression value derived from Transcriptome Analysis Console v3.0
|
Data processing |
Raw data were processed with Transcriptome Analysis Console v3.0 (Affymetrix). Data analysis and statistical evaluations were performed with R version 3.4.3. Class comparison paired T test was used with an FDR of 0.05% to 0.1%.
|
|
|
Submission date |
Feb 22, 2021 |
Last update date |
Feb 23, 2021 |
Contact name |
vanaja konduri |
E-mail(s) |
[email protected]
|
Phone |
7137986413
|
Organization name |
Baylor College of Medicine
|
Department |
Pathology&Immunology
|
Lab |
N911
|
Street address |
One Baylor Plaza
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL24539 |
Series (1) |
GSE167279 |
Exon Level Expression Profiling: a Novel Unbiased Transcriptome (Human) |
|
Relations |
Alternative to |
GSM5100782 (gene-level analysis) |