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Sample GSM5100794 Query DataSets for GSM5100794
Status Public on Feb 23, 2021
Title Donor 2-CD8+CD161- alt splice
Sample type RNA
 
Source name Peripheral blood PBMC
Organism Homo sapiens
Characteristics cells: CD8+CD161- T cells
treatment: PDAC antigen-loaded autologous DC
Treatment protocol CD8+CD161+ and CD8+CD161- T cells were magnetically sorted from healthy donor peripheral blood.
Extracted molecule total RNA
Extraction protocol RNA was isolated from the cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. Inclusion of 10 mL/L of the RNase inhibitor NucleoGuard (AmpTec) in the lysis buffer improved RNA yield and recoveries of long transcripts. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Human WT Pico-Clariom D Exon Arrays (Affymetrix) and scanned at the Sequencing and Microarray Facility at the University of Texas MDAnderson Cancer Center.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Donor 2-CD8+CD161-
RMA expression value derived from Transcriptome Analysis Console v3.0
Data processing Raw data were processed with Transcriptome Analysis Console v3.0 (Affymetrix). Data analysis and statistical evaluations were performed with R version 3.4.3. Class comparison paired T test was used with an FDR of 0.05% to 0.1%.
 
Submission date Feb 22, 2021
Last update date Feb 23, 2021
Contact name vanaja konduri
E-mail(s) [email protected]
Phone 7137986413
Organization name Baylor College of Medicine
Department Pathology&Immunology
Lab N911
Street address One Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL24539
Series (1)
GSE167279 Exon Level Expression Profiling: a Novel Unbiased Transcriptome (Human)
Relations
Alternative to GSM5100782 (gene-level analysis)

Supplementary file Size Download File type/resource
GSM5100794_1-18-18_CD8+CD161-_030718_Clariom_D_Human_.CEL.gz 20.9 Mb (ftp)(http) CEL
GSM5100794_1-18-18_CD8+CD161-_030718_Clariom_D_Human_.sst-rma-alt-splice-sst-dabg.chp.gz 13.1 Mb (ftp)(http) CHP
Processed data provided as supplementary file

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