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Status
Public on Apr 19, 2010
Title
1-10_Heterozygous-AtcesA6_Rep2_ATH1
Sample type
RNA
Source name
Col-0, heterozygous AtCesA6
Organism
Arabidopsis thaliana Characteristics
ecotype: Col-0
Extracted molecule
total RNA
Extraction protocol
Qiagen RNAeasy kit protocol
Label
biotin
Label protocol
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
Hybridization protocol
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
Scan protocol
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
Description
Room chamber, untreated 4-week-old heterozygous irx6-1 plants, 50% humidity, 22oC
Data processing
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
Submission date
Feb 08, 2010
Last update date
Apr 18, 2010
Contact name
Nottingham Arabidopsis Stock Centre (NASC)
E-mail(s)
[email protected] Phone
+44 (0)115 951 3237
Fax
+44 (0)115 951 3297
URL
http://arabidopsis.info/ Organization name
Nottingham Arabidopsis Stock Centre (NASC)
Department
School of Biosciences, University of Nottingham
Street address
Sutton Bonington Campus
City
Loughborough
ZIP/Postal code
LE12 5RD
Country
United Kingdom
Platform ID
GPL198 Series (1)
Data table header descriptions ID_REF VALUE MAS5.0-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-r2-P1-cre-5_at
8111.12353515625
P
0.000244141003349796
AFFX-r2-P1-cre-3_at
10893.4892578125
P
0.000244141003349796
AFFX-r2-Ec-bioD-5_at
2573.15502929688
P
0.000244141003349796
AFFX-r2-Ec-bioD-3_at
3044.27514648438
P
0.000244141003349796
AFFX-r2-Ec-bioC-5_at
871.093872070313
P
0.000244141003349796
AFFX-r2-Ec-bioC-3_at
793.571960449219
P
0.000244141003349796
AFFX-r2-Ec-bioB-M_at
230.232559204102
P
0.000244141003349796
AFFX-r2-Ec-bioB-5_at
232.923477172852
P
0.000244141003349796
AFFX-r2-Ec-bioB-3_at
213.221801757813
P
0.000244141003349796
AFFX-r2-Bs-thr-M_s_at
1.03993940353394
A
0.995850026607513
AFFX-r2-Bs-thr-5_s_at
1.92267251014709
A
0.828612983226776
AFFX-r2-Bs-thr-3_s_at
5.21687746047974
A
0.533936023712158
AFFX-r2-Bs-phe-M_at
4.70595645904541
A
0.780517995357513
AFFX-r2-Bs-phe-5_at
3.96877360343933
A
0.334473013877869
AFFX-r2-Bs-phe-3_at
1.91391658782959
A
0.888427972793579
AFFX-r2-Bs-lys-M_at
7.40391731262207
A
0.334473013877869
AFFX-r2-Bs-lys-5_at
0.684096395969391
A
0.928955078125
AFFX-r2-Bs-lys-3_at
14.165225982666
P
0.0239257998764515
AFFX-r2-Bs-dap-M_at
5.9624924659729
A
0.601073980331421
AFFX-r2-Bs-dap-5_at
0.582013368606567
A
0.904784977436066
22810 1073 Kbytes .Supplementary file Size Download File type/resource GSM507021.CEL.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
