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Sample GSM506992 Query DataSets for GSM506992
Status Public on May 05, 2010
Title rat hippocampus (combined dorsal and ventral aspects), aged control, subject 3
Sample type RNA
 
Source name rat hippocampus (combined dorsal and ventral aspects), aged control, subject 3
Organism Rattus norvegicus
Characteristics model: Fischer 344
gender: Male
age: 21 to 22 months old
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol reagent and following the manufacturer's RNA isolation protocol (Invitrogen, #15596). One milliliter of TRIzol solution was added to each tube containing the frozen tissue block, and the tissue was homogenized by 10 passages through an 18.5 ga syringe needle. After centrifugation, the RNA was precipitated from the aqueous layer, washed, and dissolved in RNase-free water. RNA concentration and integrity were assessed by spectrophotometry and gel electrophoresis. The RNA samples were stored at 80°C.
Label Standard Affymetrix
Label protocol Standard Affymetrix
 
Hybridization protocol Standard Affimetrix
Scan protocol Standard Affymetrix
Description -
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 07, 2010
Last update date May 05, 2010
Contact name Eric M Blalock
E-mail(s) [email protected]
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL1355
Series (1)
GSE20219 Effects of Long-term Pioglitazone Treatment on Peripheral and Central Markers of Aging

Data table header descriptions
ID_REF Probe Set
VALUE MAS5-calculated Signal intensity
DETECTION P-VALUE the probability that a probe set is absent, ranging from 0 to 1 (in this study, probe set with p < 0.05 were considered present)

Data table
ID_REF VALUE DETECTION P-VALUE
AFFX-BioB-5_at 257.5267 0.000753643
AFFX-BioB-M_at 374.1881 6.02111E-05
AFFX-BioB-3_at 197.3456 0.000126798
AFFX-BioC-5_at 736.821 8.14279E-05
AFFX-BioC-3_at 793.1919 4.42873E-05
AFFX-BioDn-5_at 1744.335 7.00668E-05
AFFX-BioDn-3_at 3042.505 0.000126798
AFFX-CreX-5_at 10668.76 5.16733E-05
AFFX-CreX-3_at 10902.8 4.42873E-05
AFFX-DapX-5_at 6.743037 0.3684377
AFFX-DapX-M_at 27.0277 0.1658614
AFFX-DapX-3_at 5.185775 0.7723636
AFFX-LysX-5_at 1.85525 0.3825893
AFFX-LysX-M_at 15.95393 0.4851099
AFFX-LysX-3_at 1.003718 0.6729205
AFFX-PheX-5_at 1.32566 0.9457867
AFFX-PheX-M_at 1.411527 0.8432685
AFFX-PheX-3_at 7.978001 0.4702405
AFFX-ThrX-5_at 8.68834 0.5886198
AFFX-ThrX-M_at 13.79644 0.3406481

Total number of rows: 31099

Table truncated, full table size 928 Kbytes.




Supplementary file Size Download File type/resource
GSM506992.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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