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Sample GSM49877 Query DataSets for GSM49877
Status Public on Dec 20, 2005
Title medial motoneurons (MMN) 9, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: medial motoneuron (MMN)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 1 - Pair 2 - MMN
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF Probeset ID
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 41.716581
100002_at 657.734483
100003_at 28.871985
100004_at 150.800358
100005_at 205.665837
100006_at 51.725275
100007_at 250.587757
100009_r_at 895.980346
100010_at 64.077179
100011_at 90.717543
100012_at 389.070819
100013_at 125.047127
100014_at 202.676997
100015_at 146.365437
100016_at 45.801732
100017_at 49.190124
100018_at 171.107101
100019_at 277.3155
100020_at 243.503262
100021_at 32.109991

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49877.CEL.gz 3.0 Mb (ftp)(http) CEL

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