|
| Status |
Public on Jan 16, 2010 |
| Title |
E2A/HEB-deficient (DKO) DP cells, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
E2A and HEB deficient double positive cells sorted from thymus of 8 week old mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: thymus
genotype: E2Aflox/flox HEBflox/flox cd4-cre+
age: 8 weeks
|
| Treatment protocol |
cells were stored in 5% BGS+ PBS prior to FACS
|
| Growth protocol |
thymuses were harvested from individual mice, single cell suspensions were made and cells were stained with antibodies for CD4 and CD8 before sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from E2A-deficient HEB-deficient double-positive thymocytes
|
| Data processing |
The data were analyzed using Expression File Creator module on Gene Pattern Suite and RMA as normalization method.
|
| |
|
| Submission date |
Jan 15, 2010 |
| Last update date |
Jan 15, 2010 |
| Contact name |
Louise D'Cruz |
| Organization name |
UCSD
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19923 |
Expression data from E protein deficient double-positive (DP) thymocytes |
|
