|
| Status |
Public on Jan 16, 2010 |
| Title |
Liver rosiglitazone 28µmol/kg technical replicate 5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pool of db/db control mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
|
| Treatment protocol |
Per os treatment for 18 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3,Cy5
|
| Label protocol |
Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
|
| |
|
| Channel 2 |
| Source name |
db/db mouse treated with 28µmol/kg of rosiglitazone
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
|
| Treatment protocol |
Per os treatment for 18 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5,Cy3
|
| Label protocol |
Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
|
| |
|
| |
| Hybridization protocol |
Samples were incubated at 60°C for 30 minutes and then hybridized for 17 hours at 65°C in a 10 rpm rotary oven. Slides were then washed in buffer 1 and 2, according to manufacturer’s specification.
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5).
|
| Description |
Analysis used the pool of db/db mice as control samples for comparison to the treated mice. Technical replicate 5 of 6. Db/db mice treated with 28µmol/kg of rosiglitazone
|
| Data processing |
Agilent Feature Extraction Software (v 9.5) was used for background subtraction and LOWESS normalization (GE2-v5_95_Feb07 protocol). Rosetta Resolver (v 7.1) was used for the dye-swap combination.
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Jan 15, 2010 |
| Contact name |
Aurélie Cotillard |
| Organization name |
Institut de Recherches Servier
|
| Street address |
125 chemin de ronde
|
| City |
Croissy-sur-Seine |
| ZIP/Postal code |
78290 |
| Country |
France |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE19896 |
Diabetic db/db mice treated with three doses of rosiglitazone and S compound |
|
