|
| Status |
Public on Jul 01, 2010 |
| Title |
Columbia.Dry.MD.rep1 |
| Sample type |
RNA |
| |
|
| Source name |
whole rosette, water-limited, midday
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: wild type
tissue: whole rosette
age: 32 days
treatment: water-limited
time of day: 12h00 (midday)
|
| Treatment protocol |
After 28 days of growth without water limitation, no further input of water was given to 50% of plants. The other 50% were watered as before.
|
| Growth protocol |
Col-0 seeds were planted on Sunshine mix #1. Seeds were cold stratified for 3 days, then transferred to growth chambers with a 12h photoperiod. Plants were watered every other morning with tap water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, as per manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol: Eukaryotic target preparation: One-cycle target labeling assay.
|
| |
|
| Hybridization protocol |
Affymetrix protocol: Eukaryotic target preparation: Eukaryotic target hybridization.
|
| Scan protocol |
Affymetrix protocol: GeneChip Scanner 3000 7G Affymetrix 00-0073.
|
| Description |
28 days without water limitation followed by 4 days without further input of water 4 days.
bot0861
|
| Data processing |
GC-RMA in BioConductor.
|
| |
|
| Submission date |
Dec 29, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Malcolm M. Campbell |
| E-mail(s) |
[email protected]
|
| Phone |
416-946-0817
|
| Fax |
416-978-5878
|
| URL |
http://www.csb.utoronto.ca/faculty/campbell-malcolm
|
| Organization name |
University of Toronto
|
| Department |
Cell & Systems Biology
|
| Lab |
3058ESC
|
| Street address |
25 Willcocks Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3B2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE19700 |
Time of day shapes the Arabidopsis drought transcriptomes |
|
| Relations |
| Reanalyzed by |
GSE118579 |
