|
| Status |
Public on Nov 30, 2020 |
| Title |
BNTX-Pf |
| Sample type |
RNA |
| |
|
| Source name |
Pf exposed to BNTX
|
| Organism |
Plasmodium falciparum |
| Characteristics |
Stage: ring
treatment: 7-benzylidenealtrexone
|
| Treatment protocol |
P. falciparum synchronized at the ring stage was cultured in the presence of dihydroartemisinin, 7-benzylidenenaltrexon, or rimonaband hydrochloride for 12 h. Parasites were isolated at the end of the incubation period, and preserved in RNAprotect Cell reagent (Qiagen).
|
| Growth protocol |
Erythrocytic Plasmodium falciparum was maintained in vitro in serum-free culture medium (GFSRPMI) in a humidified atomosphere of 5% oxygen at 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled usingGeneChip 3'IVT PLUS Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix GeneChip® Plasmodium/Anopheles Genome Array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner..
|
| Description |
Pf with BNTX
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix Power Tools 1.15.0 software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Nov 02, 2020 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Hiroko Asahi |
| E-mail(s) |
[email protected]
|
| Phone |
+81-45-503-9205
|
| Organization name |
RIKEN Center for Biosystems Dynamics Research
|
| Lab |
Laboratory for Nonnatural Amino Acid Technology
|
| Street address |
1-7-22, Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230 0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE160666 |
Expression data from Plasmodium falciparum |
|
