|
| Status |
Public on Jan 01, 2021 |
| Title |
RISP_Ad.lib_5 |
| Sample type |
SRA |
| |
|
| Source name |
Hypothalamus slice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: wild type
tissue: Hypothalamus
treatment: RISP_Ad.lib
|
| Treatment protocol |
Hypothalamus slice were collected from 4-week risperidone diet fed mice at at ad litem or pair feed condition where their body weight were not different from control diet fed mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by the Direct-zol RNA Kits (ZYMO) according to manufacturer’s recommendations and validated by the Agilent 2100 Bioanalyzer.
150 bp paired-end reads were sequenced on DNBseq platform in BGI Americas Corporation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
R7461
|
| Data processing |
We obtained averaged 2.5 million mapped reads per sample with 90% unique mapped efficiency.
The paired-end clean reads were aligned to the mouse reference genome GRCm38_mm10 (release99) using STAR and each gene reads numbers were count by FeatureCounts.
Differential expression gene (DEG) analysis was performed by DEseq2 package in which gene length and dispersion bias was corrected.
The resulting P values were adjusted using the Benjamini and Hochberg’s approach for controlling the false discovery rate (FDR).
Genes with an adjusted P value < 0.05 and logFold > 0.3 found by DESeq2 were assigned as differentially expressed.
Genome_build: mm10
Supplementary_files_format_and_content: Raw counts
|
| |
|
| Submission date |
Sep 29, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Li Li |
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Boulevard
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL28457 |
| Series (1) |
| GSE158751 |
The Atypical Antipsychotic Risperidone Targets Central Melanocortin 4 Receptors to Cause Weight |
|
| Relations |
| BioSample |
SAMN16291338 |
| SRA |
SRX9214976 |
