|
| Status |
Public on Dec 05, 2009 |
| Title |
Foreman_1-5_L16-4hrs_Rep1_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Ler, 16C
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: whole plant excluding roots
stock code: NW20
|
| Growth protocol |
Seedlings were grown on soil at 16C for 11 days, then transferred to 22C for 21 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
Seeds were suspended in water and individually pipetted onto the soil surface. Trays were stratified at 4C in the dark for 5 days. Commercial Soil: 3 parts Levington's F2 soil, 1 part sand. Growth room 16C 50%
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE19258 |
Temperature-controlled inhibition of phyB action |
|
| Relations |
| Reanalyzed by |
GSE118579 |
