|
Status |
Public on Feb 01, 2010 |
Title |
Follicular lymphoma_FL.E.10 |
Sample type |
RNA |
|
|
Source name |
Follicular lymphoma bioptic tissue
|
Organism |
Homo sapiens |
Characteristics |
cell type: Follicular lymphoma biopsy
|
Treatment protocol |
The frozen tissue was homogeneized in Trizol reagent.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's indications.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for at least 16 hr at 45C on GeneChip Human Genome Array U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
Description |
Gene expression data from follicular lymphoma
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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|
|
Submission date |
Nov 30, 2009 |
Last update date |
Nov 30, 2009 |
Contact name |
Katia Basso |
E-mail(s) |
[email protected]
|
Phone |
212-8515269
|
Fax |
212-8515256
|
Organization name |
Columbia University
|
Department |
Institute for Cancer Genetics
|
Street address |
1150 St. Nicholas Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE12195 |
Mutations of multiple genes deregulate the NF-kB pathway in diffuse large B cell lymphoma |
|