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Sample GSM476286 Query DataSets for GSM476286
Status Public on Feb 01, 2010
Title Follicular lymphoma_FL.E.10
Sample type RNA
 
Source name Follicular lymphoma bioptic tissue
Organism Homo sapiens
Characteristics cell type: Follicular lymphoma biopsy
Treatment protocol The frozen tissue was homogeneized in Trizol reagent.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's indications.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for at least 16 hr at 45C on GeneChip Human Genome Array U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from follicular lymphoma
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 30, 2009
Last update date Nov 30, 2009
Contact name Katia Basso
E-mail(s) [email protected]
Phone 212-8515269
Fax 212-8515256
Organization name Columbia University
Department Institute for Cancer Genetics
Street address 1150 St. Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL570
Series (1)
GSE12195 Mutations of multiple genes deregulate the NF-kB pathway in diffuse large B cell lymphoma

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 399.5
1053_at 617.4
117_at 216
121_at 1096.3
1255_g_at 79.7
1294_at 1345.7
1316_at 148.7
1320_at 29.8
1405_i_at 1072.5
1431_at 108.9
1438_at 35.4
1487_at 536.8
1494_f_at 116.4
1552256_a_at 954.1
1552257_a_at 754.4
1552258_at 255.5
1552261_at 216.7
1552263_at 545.2
1552264_a_at 939.2
1552266_at 254.6

Total number of rows: 54675

Table truncated, full table size 873 Kbytes.




Supplementary file Size Download File type/resource
GSM476286.CEL.gz 8.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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