|
| Status |
Public on Oct 20, 2021 |
| Title |
MOLM-13.WM1119_H3K9ac_no1 |
| Sample type |
SRA |
| |
|
| Source name |
MOLM-13
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: WM-1119
cell line: MOLM-13
antibody: H3K9ac
|
| Treatment protocol |
MOLM-13 cells were treated with 4uM WM-1119 for 4 days.
|
| Growth protocol |
MOLM-13 cell line was cultured in RPMI (Corning, 10-041-CV) with 10% FBS (Life Technologies, 16000044) and 1% Penicillin Streptomycin (Gibco, 15-140-122).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seq samples were prepared following standard protocol in this manuscript.
ChIP-seq libraries were prepared following the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) protocol, using NEBNext Multiplex Oligos Index Primers Sets
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
alignment using Bowtie2 to generate sam files
sam and bam files were processed with SAMtools
eak calling using MACS2
peaks were merged with BEDTools
read counts were calculated and normalized in the merged peaks for every sample
Genome_build: hg38
Supplementary_files_format_and_content: normalized count for each peaks across samples
|
| |
|
| Submission date |
Aug 28, 2020 |
| Last update date |
Oct 20, 2021 |
| Contact name |
Jinyang Li |
| E-mail(s) |
[email protected]
|
| Phone |
2155885072
|
| Organization name |
The Rockefeller University
|
| Lab |
Daniel Mucida Lab
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL21697 |
| Series (1) |
| GSE157042 |
Epigenetic profiling of cells following WM-1119 treatment |
|
| Relations |
| BioSample |
SAMN15930465 |
| SRA |
SRX9029187 |
