|
| Status |
Public on Nov 20, 2009 |
| Title |
wild type, untreated, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type, untreated leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: wild type
tissue: leaf
age: three weeks
treatment: untreated
|
| Treatment protocol |
The leaves of wild type and mutant plants were or were not infiltrated with a Pst suspension at the concentration of 10-5 cfu ml-1.
|
| Growth protocol |
The seedlings were grown in a growth chamber with a short day (8h light) illumination at 20C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol/chloroform extraction and LiCl purification of total RNA were performed.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6-10 ug total RNA. For the detailed protocol, please check the website http://www.csb.utoronto.ca/resources/facilities/affymetrix-genechip.
|
| |
|
| Hybridization protocol |
Prehyb, hybridisation and wash were performed by Bio-Array Resource (BAR) center, University of Toronto (http://www.csb.utoronto.ca/resources/facilities/affymetrix-genechip).
|
| Scan protocol |
The scannings were performed by Bio-Array Resource (BAR) center, University of Toronto (http://www.csb.utoronto.ca/resources/facilities/affymetrix-genechip).
|
| Description |
Gene expression data from 3-week-old wild type leaves.
bot0715
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 19, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Yangdou Wei |
| Organization name |
University of Saskatchewan
|
| Department |
Biology
|
| Street address |
112 Sceince Place
|
| City |
Saskatoon |
| State/province |
SK |
| ZIP/Postal code |
S7N 5E2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE19109 |
Differential gene expression profiling from Arabidopsis thaliana wild type (Columbia-0) and lht1 mutant leaves |
|
| Relations |
| Reanalyzed by |
GSE118579 |
