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Sample GSM4731429 Query DataSets for GSM4731429
Status Public on Nov 02, 2020
Title KC rep 3
Sample type RNA
 
Source name KC rep 3
Organism Mus musculus
Characteristics type of cell: Kupffer control cells
Extracted molecule total RNA
Extraction protocol Trizol standard RNA extraction. An additional cleaning up of RNA followed using the miRNeasy Kit (Qiagen, Hilden, Germany).
Integrity and quality of RNA was checked with the Agilent 2100 Bioanalyzer platform (Agilent Technologies).
Label biotin
Label protocol Labelling was performed following the standard Agilent Protocol for the miRNA Microarray System with a
miRNA Complete Labelling, including Agilent miRNA Spike-ins.
 
Hybridization protocol The hybridized arrays were washed, dried and finally scanned on using an Agilent Microarray Scanner G2565CA (Agilent Technologies, USA).
Scan protocol The Agilent’s Microarray Scanner Sytem (Agilent Technologies) was used for detecting fluorescence signals on the hybridized microarrays.
The microarray image files were processed with the Agilent Feature Extraction Software (FES),
Description replicate 3
Data processing The microarray raw data were normalized using the quantile method.
 
Submission date Aug 18, 2020
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) [email protected]
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL16199
Series (2)
GSE156430 An Integrative Omics Approach Reveals Involvement of BRCA1 in Hepatic Metastatic Progression of Colorectal Cancer (microRNA)
GSE156431 An Integrative Omics Approach Reveals Involvement of BRCA1 in Hepatic Metastatic Progression of Colorectal Cancer

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
ID_REF VALUE
1 4.383603e+002
2 -2.911881e+001
3 -2.447150e+001
4 -2.860057e+001
5 -3.054827e+001
6 -2.873460e+001
7 -3.311504e+001
8 -2.801756e+001
9 -3.129359e+001
10 -2.863288e+001
11 -1.641755e+000
12 -1.773411e+000
13 1.883321e+000
14 -6.243365e+000
16 -1.594777e+000
17 -3.346360e+000
18 3.847574e+000
21 4.929515e+000
23 -1.737826e+000
24 3.678655e+001

Total number of rows: 49324

Table truncated, full table size 978 Kbytes.




Supplementary file Size Download File type/resource
GSM4731429_KC-rep3.txt.gz 7.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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