|
| Status |
Public on Nov 02, 2020 |
| Title |
ET rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
ET rep 2
|
| Organism |
Mus musculus |
| Characteristics |
type of cell: Enthothelial tumour microenvironment (TME) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol standard RNA extraction. An additional cleaning up of RNA followed using the miRNeasy Kit (Qiagen, Hilden, Germany).
Integrity and quality of RNA was checked with the Agilent 2100 Bioanalyzer platform (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Labelling was performed following the standard Agilent Protocol for the miRNA Microarray System with a
miRNA Complete Labelling, including Agilent miRNA Spike-ins.
|
| |
|
| Hybridization protocol |
The hybridized arrays were washed, dried and finally scanned on using an Agilent Microarray Scanner G2565CA (Agilent Technologies, USA).
|
| Scan protocol |
The Agilent’s Microarray Scanner Sytem (Agilent Technologies) was used for detecting fluorescence signals on the hybridized microarrays.
The microarray image files were processed with the Agilent Feature Extraction Software (FES),
|
| Description |
replicate 2
|
| Data processing |
The microarray raw data were normalized using the quantile method.
|
| |
|
| Submission date |
Aug 18, 2020 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
[email protected]
|
| Phone |
+34 943 00 6108
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Lab |
Computational Biology and Bionformatics
|
| Street address |
Rogentstrasse
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16199 |
| Series (2) |
| GSE156430 |
An Integrative Omics Approach Reveals Involvement of BRCA1 in Hepatic Metastatic Progression of Colorectal Cancer (microRNA) |
| GSE156431 |
An Integrative Omics Approach Reveals Involvement of BRCA1 in Hepatic Metastatic Progression of Colorectal Cancer |
|
