|
Status |
Public on Jul 28, 2020 |
Title |
T1-3 |
Sample type |
RNA |
|
|
Source name |
Renal Cell Carcinoma
|
Organism |
Homo sapiens |
Characteristics |
metastatic: Yes tumor stage: 1 gender: M tissue: Renal Cell Carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RecoverAllTM Total Nucleic Acid Isolation Kit according to manufacturer’s instructions
|
Label |
TaqMan
|
Label protocol |
Quantification of miRNA was done using the TaqMan OpenArray, which profiles 754 known miRNAs using qPCR
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
microRNA
|
Data processing |
A Ct threshold of 30 was used for detectable level of expression. MiRNAs were further filtered by removing those with no expression in any samples and samples were filtered that had < 10% of miRNAs detected. Housekeeping miRNAs were the top 10 most stable miRNAs from the NormqPCR package_x0001_, which were used to normalize raw Ct values using ∆Ct normalization Differential expression was calculated using limma Matrix normalized worksheet reports normalized signal (against 10 most stable housekeeping genes).
|
|
|
Submission date |
Jul 27, 2020 |
Last update date |
Jul 28, 2020 |
Contact name |
Andrew Shih |
E-mail(s) |
[email protected]
|
Phone |
516-562-2960
|
Organization name |
Feinstein Institute - Northwell Health
|
Street address |
350 Community Dirive
|
City |
Mahasset |
State/province |
NY |
ZIP/Postal code |
11030 |
Country |
USA |
|
|
Platform ID |
GPL18942 |
Series (2) |
GSE155209 |
Prognostic Molecular Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas |
GSE155210 |
Prognostic Molecular Signatures and Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas |
|