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Sample GSM4697409 Query DataSets for GSM4697409
Status Public on Jul 28, 2020
Title T1-3
Sample type RNA
 
Source name Renal Cell Carcinoma
Organism Homo sapiens
Characteristics metastatic: Yes
tumor stage: 1
gender: M
tissue: Renal Cell Carcinoma
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RecoverAllTM Total Nucleic Acid Isolation Kit according to manufacturer’s instructions
Label TaqMan
Label protocol Quantification of miRNA was done using the TaqMan OpenArray, which profiles 754 known miRNAs using qPCR
 
Hybridization protocol n/a
Scan protocol n/a
Description microRNA
Data processing A Ct threshold of 30 was used for detectable level of expression.
MiRNAs were further filtered by removing those with no expression in any samples and samples were filtered that had < 10% of miRNAs detected.
Housekeeping miRNAs were the top 10 most stable miRNAs from the NormqPCR package_x0001_, which were used to normalize raw Ct values using ∆Ct normalization
Differential expression was calculated using limma
Matrix normalized worksheet reports normalized signal (against 10 most stable housekeeping genes).
 
Submission date Jul 27, 2020
Last update date Jul 28, 2020
Contact name Andrew Shih
E-mail(s) [email protected]
Phone 516-562-2960
Organization name Feinstein Institute - Northwell Health
Street address 350 Community Dirive
City Mahasset
State/province NY
ZIP/Postal code 11030
Country USA
 
Platform ID GPL18942
Series (2)
GSE155209 Prognostic Molecular Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas
GSE155210 Prognostic Molecular Signatures and Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas

Supplementary data files not provided
Processed data are available on Series record

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