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| Status |
Public on Jul 02, 2021 |
| Title |
EPCR+ HSC 35 |
| Sample type |
SRA |
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| Source name |
cord blood
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| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34+ cells
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
C1 Fluidigm
Multiplexed Nextera XT; each single cell was barcoded
scRNA-seq 3 million 100 bp pair-end reads per cell
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
AFO554A301_S139_L005
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| Data processing |
Sequencing was set for 3 million 100 bp pair-end reads per cell under Illumina’s kit instructions.
Raw sequencing reads were mapped with STAR
Expression counts estimates and transcripts per million (TPM) were generated using RSEM
Genome_build: GRCh38
Supplementary_files_format_and_content: transcripts per million in txt matrix from
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| Submission date |
Jul 27, 2020 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
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| Department |
DKTK Frankfurt
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| Street address |
Theodor-Stern-Kai 7
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| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platform ID |
GPL20301 |
| Series (1) |
| GSE155174 |
Single Cell analysis of the most primitive Human Hematopoietic Stem Cell Population. |
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| Relations |
| BioSample |
SAMN15650689 |
| SRA |
SRX8831120 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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