|
| Status |
Public on Jul 01, 2021 |
| Title |
CD90+CD49f+ HSC 69 |
| Sample type |
SRA |
| |
|
| Source name |
CD90+CD49f+ HSC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cord blood
cell type: CD90+CD49f+ HSC
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
0.2% TrixonX-100 supplemented with 1 U/ul of RNAse inhibitor
Multiplexed Nextera XT; each single cell was barcoded then pooled per plate.
scRNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
S1-P6-G02
|
| Data processing |
Sequencing was set for 3 million 100 bp paar-end reads per cell under Illumina’s kit instructions.
Raw sequencing reads were mapped with STAR
Expression counts estimates and transcripts per million (TPM) were generated using RSEM
Genome_build: GRCh38
Supplementary_files_format_and_content: raw counts (TPM) in txt matrix from
|
| |
|
| Submission date |
Jul 22, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE154931 |
Single Cell analysis of the two Human Hematopoietic Stem Cell Populations |
|
| Relations |
| BioSample |
SAMN15609149 |
| SRA |
SRX8803029 |
