|
| Status |
Public on Nov 04, 2009 |
| Title |
ELK1-siRNA transfected cells vs. mock transfected cells-siRNA1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MDA-MB-231 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
transfection: 72hr post-transfection with ELK1-siRNA
|
| Growth protocol |
MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
|
| |
|
| Channel 2 |
| Source name |
MDA-MB-231 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
transfection: 72hr after mock-transfection
|
| Growth protocol |
MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized using Agilent's gene expression hybridization kit and wash buffers according to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5).
|
| Description |
ELK1 knock-down vs control
|
| Data processing |
Agilent Feature Extraction Software (v 9.5) was used for data extraction.
|
| |
|
| Submission date |
Nov 02, 2009 |
| Last update date |
Nov 03, 2009 |
| Contact name |
Hani Goodarzi |
| Organization name |
UCSF
|
| Department |
Biochemistry and Biophysics
|
| Street address |
600 16th St, GH S312D
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE18849 |
ELK1 and NFYA knockdown in MBA-MD-231 cell line |
| GSE18874 |
Decoy vs Scrambled and siRNA vs Mock Transfected MBA-MD-231 Cell Line |
|
