|
| Status |
Public on Aug 20, 2020 |
| Title |
6_Thor_Hyper_induced_-FBS_+Rapa_replicate1 |
| Sample type |
SRA |
| |
|
| Source name |
late stage embryo-derived S2 cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
transfected plasmids: pAC5.1-eGFP and pMTA-thor-hyperADARcd
expression of gene in pmta: Induced the expression overnight
treatment: Serum depletion and 50nM of Rapamycin for 5 hrs
|
| Growth protocol |
S2 cells were grown in Hyclone SFX-insect media with 10% of FBS. PC3 cells were grown in F-12K Medium (ATCC) with 10% of FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GFP-positive cells were sorted and collected with a BD FACSAria II machine. Total RNA was extracted with TRIzol LS reagent.
TRIBE: Standard Illumina TruSeq RNA Library Kit.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
TRIBE, RNA-seq
Poly(A) tailed RNA
|
| Data processing |
Library strategy: TRIBE
Trimmomatic-0.30/trimmomatic-0.30.jar SE -phred33 CROP:70 HEADCROP:6 LEADING:25 TRAILING:25 AVGQUAL:$avgquality MINLEN:19
Genome_build: Drosophila dm3 (BDGP Release 5); Human hg38 (GRCh38)
Supplementary_files_format_and_content: *.threshold.txt: Edited sites
Supplementary_files_format_and_content: *bw: bigWig files
|
| |
|
| Submission date |
Jun 26, 2020 |
| Last update date |
Aug 20, 2020 |
| Contact name |
Hua Jin |
| E-mail(s) |
[email protected]
|
| Phone |
86-18500905853
|
| Organization name |
Beijing Institute of Technology
|
| Street address |
Rm 225-1, Bldg Qiushilou, Beijing Institute of Technology,
|
| City |
Beijing |
| State/province |
Choose a State or Province |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE153346 |
TRIBE editing reveals specific mRNA targets of eIF4E-BP in Drosophila and in mammals |
|
| Relations |
| BioSample |
SAMN15376574 |
| SRA |
SRX8622788 |
