|
| Status |
Public on Jun 18, 2020 |
| Title |
hsp70-4c-5h-2 |
| Sample type |
SRA |
| |
|
| Source name |
Drosophila adult head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: hsp70-4c
time point: 5 hours (short-term)
tissue: adult head
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
For each behavioural experiment, four biological replicates of approximately 100 flies were collected and frozen. Naive or trained males snap-frozen in liquid nitrogen at the appropriate time point after training were manually decapitated. The heads were collected on a dry ice-cooled surface. Total RNA extraction was performed with RNAzol reagent (Molecular Research Center, USA) according to the manufacturer’s protocol
Libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer’s guidelines. The 75-bp single-end sequencing was conducted on an Illumina NextSeq 500 platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Short read trimming (Trimmomatic)
alignment of Illumina short reads with STAR_2.5.0a
Read counting with featureCounts (v1.5.0-p1)
Genome_build: Drosophila melanogaster dm6
Supplementary_files_format_and_content: Tab-delimeted text file with FlyBase gene identifyer (1 column) and read counts per gene (2 column)
|
| |
|
| Submission date |
Jun 17, 2020 |
| Last update date |
Jun 18, 2020 |
| Contact name |
Alexander Pertovich Rezvykh |
| E-mail(s) |
[email protected]
|
| Organization name |
Engelhardt Molecular Biology Institute
|
| Department |
Russian Academy of Sciences
|
| Lab |
Laboratory of molecular mechanisms of biological adaptation
|
| Street address |
Vavilova, 32
|
| City |
Moscow |
| State/province |
Moscow |
| ZIP/Postal code |
119991 |
| Country |
Russia |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE152647 |
Hsp70 affects memory formation and behaviourally relevant gene expression in Drosophila melanogaster. |
|
| Relations |
| BioSample |
SAMN15295715 |
| SRA |
SRX8564090 |
