|
| Status |
Public on Oct 03, 2009 |
| Title |
p300+/- Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampal tissue from heterozygous p300 mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype: p300+/-
replicate: 1
strain: C57BL/6
gender: female
age: adult
tissue: hippocampus
|
| Treatment protocol |
The brains were immediately removed, and hippocampi rapidly dissected and placed in RNAlater solution (Qiagen, Venlo, The Netherlands) until total RNA extraction.
|
| Growth protocol |
P300 deficient heterozygous mice were maintained in a C57BL/6J pure background. Mice were group-housed in single-sex cages on a light:dark cycle (12/12 h) with food and water available ad limitum. The wild type mice used as control were, in all cases, littermates of the mutant mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted separately from individual hippocampi using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) per manufacturers instructions followed by a cleanup step using RNeasy RNA purification columns (Qiagen, Valencia, CA, USA). Equal amounts of total RNA from four animals were pooled, processed, and hybridized to Mouse Genome 430 2.0 genechips (Affymetrix, Santa Clara, CA). Three independent biological replicates were prepared for each genotype (wild-type and p300 deficient mice (p300+/-)). Mouse Genome 430 2.0 genechips were hybridized, stained, washed and screened for quality according to the manufacturer's protocol. Microarray data were processed, normalized and statistically analyzed using GeneSpring GX (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Following Affymetrix instructions
|
| |
|
| Hybridization protocol |
Following Affymetrix instructions
|
| Scan protocol |
Following Affymetrix instructions
|
| Description |
Gene expression data from hippocampal tissue
|
| Data processing |
GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of all samples. Data are represented as log2.
|
| |
|
| Submission date |
Oct 02, 2009 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Angel Barco |
| Organization name |
Instituto de Neurociencias (UMH-CSIC)
|
| Street address |
Av. Santiago Ramón y Cajal
|
| City |
Sant Joan d'Alacant |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18383 |
Syndromic features and mild cognitive impairment in mice with genetic reduction on p300 activity |
|
