|
| Status |
Public on Sep 28, 2010 |
| Title |
an3-4 leaf primordia rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis leaf primordia
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
phenotype: an3-4 mutants
|
| Biomaterial provider |
Rikkyo University
|
| Treatment protocol |
Leaf primordia were transferred to liquid nitrogen before RNA extraction
|
| Growth protocol |
Plants were grown at 22C under continuous light.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard affymetrix protocol
|
| Scan protocol |
Standard affymetrix protocol
|
| Description |
Gene expression data of the first set of leaf primorida harvested 7 days after sowing.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 29, 2009 |
| Last update date |
Sep 29, 2009 |
| Contact name |
Gorou Horiguchi |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3985-4146
|
| Fax |
+81-3-3985-4146
|
| Organization name |
Rikkyo University
|
| Department |
College of Science, Faculty of Life Science
|
| Street address |
3-34-1, Nishi-Ikebukuro, Toshima-ku
|
| City |
Tokyo |
| ZIP/Postal code |
171-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE18311 |
Comparison of gene expression in wild type and angustifoila3 leaf primorida |
|
