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Status |
Public on May 21, 2021 |
Title |
Com(SC+LC)-2: composite lymphomas including both the large and small-cell components 2 |
Sample type |
genomic |
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Source name |
gastric resection
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Organism |
Homo sapiens |
Characteristics |
gender,age, tissue type: F, 74, cryopreserved gender: F age: 74 tissue: composite lymphomas including both the large and small-cell components tissue type: cryopreserved
|
Extracted molecule |
genomic DNA |
Extraction protocol |
A tumor cell content of at least 70% was ensured based on morphologic inspection in every sample analyzed herein. Extraction of DNA from FFPE tissue was performed using the truXTRAC FFPE DNA micro Tube Kit (Covaris, USA) . DNA extraction from cryo-preserved material was performed using the QIAamp DNA Mini Kit (Qiagen, Germany).
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Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
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Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium MethylationEPIC BeadChip
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Scan protocol |
Arrays were imaged using iScan using standard recommended Illumina scanner setting
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Data processing |
GenomeStudio software (v2011.1; methylation module 1.9.0) GenomeStudio software (v2011.1; methylation module 1.9.0) normalized avg. beta values. For data normalization, the intrinsic controls present on the array were used.
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Submission date |
May 12, 2020 |
Last update date |
May 21, 2021 |
Contact name |
Thomas FE Barth |
E-mail(s) |
[email protected]
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Organization name |
Ulm University
|
Department |
Pathology
|
Street address |
Albert-Einstein-Allee 11
|
City |
Ulm |
State/province |
Select... |
ZIP/Postal code |
89081 |
Country |
Germany |
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Platform ID |
GPL21145 |
Series (1) |
GSE150417 |
Genome-wide DNA Methylation Analysis along the Progression of Gastric Marginal Zone B-cell Lymphoma of MALT Type |
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