|
| Status |
Public on Sep 18, 2009 |
| Title |
HD, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
7-day-old seedlings grown on H2O media and transferred to 30% 2H2O media
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
age: 7-day
tissue: seedling
ecotype: Columbia
|
| Treatment protocol |
Seedlings were collected and frozen in liquid nitrogen immediately. Seedlings were ground to powder under liquid nitrogen just before total RNA isolation.
|
| Growth protocol |
Seedlings were germinated on semi-solid ATS media with or without 30% 2H2O and grown under continuous light at 22±2℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy plant mini kit (Qiagen), following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation,15 ug of cRNA were hybridized to the Affymetrix Ath121501 chip according to the protocols provided by the manufacture,GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip 3000 scanner.7G
|
| Description |
Gene expression data from 7-day-old seedlings grown on H2O media and transferred to 30% 2H2O media
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 17, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
William M. Gray |
| E-mail(s) |
[email protected]
|
| Phone |
612 624-3042
|
| Organization name |
University of Minnesota
|
| Department |
Plant Biology
|
| Street address |
1445 Gortner Ave
|
| City |
St. Paul |
| State/province |
MN |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE18153 |
Effect of deuterium oxide on Arabidopsis gene expression |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
