|
| Status |
Public on Apr 28, 2021 |
| Title |
spinal cord neurospheres in dedifferentiation condition with EGF and FGF2, mouse 2 |
| Sample type |
RNA |
| |
|
| Source name |
neurosphere culture derived from spinal cord, dedifferentiation condition
|
| Organism |
Mus musculus |
| Characteristics |
tissue: neurosphere culture
passage: passage 1
|
| Growth protocol |
neurospheres derived from the adult spinal cord and differentiated on P-D-Lysin/Laminin coated coverslip for 3 days without growth factors and in the presence of fetal bovine serum 2%., then treated for 3 days with EGF, FGF2 and Heparin after fetal bovine serum removal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction was performed with ReliaPrep RNA cell Miniprep System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on MoGene-2_1-st and HT_MG-430_PM mouse microarrays for cultures and microdissected ependymal region respectively
|
| Scan protocol |
The microarrays were scanned using the Affymetrix Gene Atlas scanner.
|
| Data processing |
The data files were generated with Affymetrix Expression Console v1.2.1 and gene expression data were normalized with RMA algorithm. Gene expression profiles were analyzed using the Affymetrix TAC 4.0 software
|
| |
|
| Submission date |
Apr 30, 2020 |
| Last update date |
May 01, 2021 |
| Contact name |
jean-philippe hugnot |
| E-mail(s) |
[email protected]
|
| Organization name |
inserm inm u1191
|
| Lab |
brain plasticity, stem cell and glial tumors
|
| Street address |
IGF INSERM UM 141 rue de la cardonille
|
| City |
MOntpellier |
| State/province |
languedoc |
| ZIP/Postal code |
34094 |
| Country |
France |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE149669 |
RNA profiling of mouse spinal cord ependymal stem cells in vivo and in vitro in different conditions |
|
