|
| Status |
Public on Mar 07, 2016 |
| Title |
PD1_3 |
| Sample type |
RNA |
| |
|
| Source name |
buffy coats of peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cell
treatment: 12 hrs culture, then 8 hrs CD3CD28 activation + PD1
|
| Treatment protocol |
immediately after isolation, cells were lysed in Trizol reagent or activated with CD3, Cd3CD28, CD3IL2
|
| Growth protocol |
freshly isolated CD4+ T cells and CD4+ T cells separated into CD25 negativ and positiv subpopulation activated with variuos conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Sep 02, 2009 |
| Last update date |
Mar 07, 2016 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2507 |
| Series (2) |
| GSE17946 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV [CD4+ T activation] |
| GSE52185 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV |
|
