|
| Status |
Public on Apr 20, 2021 |
| Title |
MG_Cx43_f_f_pre |
| Sample type |
RNA |
| |
|
| Source name |
Control, pre-immunized stage
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Isolated microglial cells
genotype: Control
Stage: pre-immunized stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT Pico Reagent Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Clariom D Assay Mouse array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Affymetrix scanner.
|
| Description |
Control sample from pre-immunized stage
|
| Data processing |
The raw data were normalized with SST-RMA algorithm (gene level) using Affymetrix Expression Console 1.4.1 software.
|
| |
|
| Submission date |
Apr 20, 2020 |
| Last update date |
Apr 21, 2021 |
| Contact name |
Ryo Yamasaki |
| E-mail(s) |
[email protected]
|
| Phone |
+81926425340
|
| Organization name |
Kyushu University
|
| Department |
Neurology
|
| Street address |
3-1-1 Maidashi, Higashi-Ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20258 |
| Series (2) |
| GSE148931 |
Gray but not white matter astroglia-specific connexin 43 ablation ameliorates neuroinflammation [Affymetrix] |
| GSE148932 |
Gray but not white matter astroglia-specific connexin 43 ablation ameliorates neuroinflammation |
|
