|
| Status |
Public on May 17, 2010 |
| Title |
mouse pharyngeal arch_E10.5_WT_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse Second heart field-containing tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129P2/OlaHsd backcrossed to C57BL/6 for 2 generations
stage: E10.5
tissue: Second heart field-containing tissue
|
| Growth protocol |
Mice were housed in the Medical University of South Carolina animal facility using institutionally approved protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pharyngeal arch-containing tissue was collected by dissection from wild-type embryos at E10.5. RNA was prepared from individual tissue isolates by Trizol extraction and purification with the RNeasy Mini kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA (10 ng) was converted into biotin-labeled cDNA suitable for hybridization to Affymetrix GeneChips (Affymetrix, Santa Clara, CA) using the WT-Ovation™ Pico amplification kit and the FL-Ovation™ cDNA Biotin V2 kit (NuGen, San Carlos, CA).
|
| |
|
| Hybridization protocol |
Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
|
| Scan protocol |
Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
|
| Description |
WT1_031307_SA_MOE430A_031307
|
| Data processing |
Scanned GeneChips (CEL files) were processed with Affymetrix GCOS to generate CHP files.
|
| |
|
| Submission date |
Sep 02, 2009 |
| Last update date |
May 17, 2010 |
| Contact name |
Jeremy Barth |
| E-mail(s) |
[email protected]
|
| Organization name |
MUSC
|
| Street address |
173 Ashley Ave, BSB 627
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425-5080 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (2) |
| GSE17934 |
Analysis of gene expression in the mouse embryo pharyngeal arch |
| GSE17936 |
Nkx2.5 regulates Jarid2 during outflow tract morphogenesis |
|
