|
| Status |
Public on Sep 21, 2010 |
| Title |
wnk 8-1 untreated, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
wnk8-1 mutant
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedlings
genotype/variation: wnk8-1 mutant
age: 2-week-old
|
| Treatment protocol |
Untreated.
|
| Growth protocol |
Arabidopsis plants were grown at 22 °C under short day conditions (8 hr light/16 hr dark)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from seedlings using TRIzol Reagenet (Invitrogen) and further purified with RNeasy Plant mini kit columns (Qiagen) according to the protocols supplied by the manufacturers.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard 1 cycle Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manua, REV3, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 with 7G upgrade
|
| Description |
Gene expression data from 2-week-old seedlings grown under short day conditions
|
| Data processing |
Data was processed in the statistical programming environment R using Bioconductor packages. Raw expression values were normalized using the robust multichip averaging algorithm.
|
| |
|
| Submission date |
Aug 04, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Tokuji Tsuchiya |
| E-mail(s) |
[email protected]
|
| URL |
http://www.cepceb.ucr.edu/members/eulgem.htm
|
| Organization name |
University of California at Riverside
|
| Department |
Botany and Plant Sciences
|
| Lab |
Thomas Eulgem's lab
|
| Street address |
3220 Batchelor Hall, University of California at Riverside
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE17499 |
Expression data from 2-week-old Arabidopsis untreated seedlings grown under a short day condition |
|
| Relations |
| Reanalyzed by |
GSE118579 |
