|
| Status |
Public on Aug 04, 2009 |
| Title |
Lung Adenocarcinoma #25 |
| Sample type |
RNA |
| |
|
| Source name |
Lung Adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: lung adenocarcinoma
tissue: tumor
grading: G3
age (years at surgery): 64
sex (m=male; f=female): F
tumor stage (according to tnm classification 2002): 2
nodal status (according to tnm classification 2002): 2
distant metastasis (according to tnm classification 2002): 1
resection status (according to tnm classification 2002): 0
smoking status (n.a.=data not available): yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each frozen tumor specimen using the Qiashredder (Qiagen, Hilden, Germany) and the RNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were generated according to the manufacturer’s protocol (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Targets were fragmented at 94°C for 35 minutes and 15µg of it was hybridized to HG-U133A chips 45°C for 16 hours. The arrays were washed and stained with 10µg/ml streptavidin-phycoerythrin. After signal amplification with biotinylated anti-streptavidin antibodies.
|
| Scan protocol |
The arrays were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from lung adneocarcinoma
|
| Data processing |
Microarray Suite 5.0 software provided by Affymetrix was used for data acquisitionFollowing data acquisition, the scanned images were quantified according to the Affymetrix GeneChip Manual by using the Data Mining Tool (DMT) 2.0, and Microarray Database software.
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Aug 18, 2014 |
| Contact name |
Jens Neumann |
| E-mail(s) |
[email protected]
|
| Phone |
0049-89-2180-73634
|
| Fax |
0049-89-2180-73671
|
| URL |
http://www.pathologie.med.uni-muenchen.de/index.html
|
| Organization name |
Ludwig-Maximilians-Universität München
|
| Department |
Institute of Pathology
|
| Street address |
Thalkirchner Straße 36
|
| City |
Munich |
| ZIP/Postal code |
80337 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE17475 |
Expression data from lung adenocarcinoma |
|
| Relations |
| Reanalyzed by |
GSE60486 |
