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Sample GSM434734 Query DataSets for GSM434734
Status Public on Aug 03, 2009
Title Exp-WT-rep1
Sample type RNA
 
Source name Exponential phase culture of EDL933 wild type in LB at OD600 of 0.3
Organism Escherichia coli O157:H7 str. EDL933
Characteristics growth phase: Exponential
genotype: WT
Growth protocol Cells were grown in Luria Bertani (LB) media at 37C at 200 rpm. Overnight cultures were inoculated with single, independent colonies in triplicate, incubated aerobically overnight at 37oC, and sub-cultured to a starting OD600 of 0.0001. Cultures were harvested at OD600 = 0.3 in exponential phase and at OD600 = 1.5 in stationary phase.
Extracted molecule total RNA
Extraction protocol Acidic Hot Phenol
Label biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Description no additional information
Data processing GCRMA method
 
Submission date Jul 30, 2009
Last update date Aug 03, 2009
Contact name Herb E Schellhorn
E-mail(s) [email protected]
Phone 9055259140-27316
Organization name McMaster University
Department Biology
Lab 405
Street address 1280 Main St. W.,
City Hamilton
State/province ON
ZIP/Postal code L8S4K1
Country Canada
 
Platform ID GPL3154
Series (1)
GSE17420 Global effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933

Data table header descriptions
ID_REF
VALUE GCRMA normalized signal intensity

Data table
ID_REF VALUE
1759068_at 2.2
1759069_at 2.2
1759070_s_at 2.1
1759071_s_at 2.1
1759072_s_at 9.7
1759073_at 2.2
1759074_at 2.2
1759075_at 2.2
1759076_s_at 2.2
1759077_s_at 8.0
1759078_at 2.2
1759079_at 2.2
1759080_s_at 7.3
1759081_s_at 2.3
1759082_s_at 2.4
1759083_at 2.4
1759084_s_at 2.2
1759085_at 2.1
1759086_s_at 2.4
1759087_s_at 7.2

Total number of rows: 10208

Table truncated, full table size 166 Kbytes.




Supplementary file Size Download File type/resource
GSM434734.CEL.gz 937.9 Kb (ftp)(http) CEL
Processed data included within Sample table
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