|
| Status |
Public on Jun 29, 2020 |
| Title |
scRNA_Male_WBL_02 |
| Sample type |
SRA |
| |
|
| Source name |
whole blood leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: whole blood leukocytes
description for exp id: 190410_0229_s05
lab data id: s5_190731_M4_B2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole blood leukocytes were isolated using a red blood cell depletion kit (Erythroclear Red Blood Cell Depletion Reagent kit; STEMCELL Technologies, cat. no. 01738)
Isolated whole blood leukocytes were encapsulated using microfluidics technology, and barcoded using a unique molecular identifier (UMI)
scRNAseq data were generated on Illumina HiSeq 3000 instruments according to the manufacturer's instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Raw sequencing data were processed with Cell Ranger software (version 3.0.0 or 3.0.2)
raw_feature_bc_matrix files were loaded to R/3.5.3 and analysis with Seurat v3.0
Genome_build: GrCh38
|
| |
|
| Submission date |
Feb 13, 2020 |
| Last update date |
Feb 22, 2023 |
| Contact name |
Mariana J. Kaplan |
| Organization name |
NIH/NIAMS
|
| Department |
Systemic Autoimmunity Branch
|
| Lab |
Lupus Clinical Trials Unit
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (2) |
| GSE145230 |
Sex differences in neutrophil biology modulate response to type I Interferons and immunometabolism (scRNA-seq) |
| GSE145231 |
Sex differences in neutrophil biology modulate response to type I Interferons and immunometabolism |
|
| Relations |
| BioSample |
SAMN14091532 |
| SRA |
SRX7716767 |
