|
| Status |
Public on Jul 15, 2009 |
| Title |
Disc Tissue 10- 28s |
| Sample type |
RNA |
| |
|
| Source name |
Disc Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
senescent or non-senescent: senescent
tissue grade: III
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected via laser capture mircodissection (LCM) and total RNA was isolated according to the Paradise reagent system.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned once via the Affymetrix 3000G scanner.
|
| Description |
Disc Tissue 10- 28s
|
| Data processing |
The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
|
| |
|
| Submission date |
Jul 14, 2009 |
| Last update date |
Jul 14, 2009 |
| Contact name |
Helen Gruber |
| E-mail(s) |
[email protected]
|
| Organization name |
Carolinas HealthCare System
|
| Department |
Orthopaedic Surgery
|
| Lab |
Orthopaedic Research
|
| Street address |
1542 Graden Terrace
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE17077 |
Gene Expression Data from Senescent vs Non-senescent Cells in the in Vivo Human Annulus |
|
