|
| Status |
Public on Nov 22, 2021 |
| Title |
Thin Tail replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Iranian native Sheep breed
|
| Organism |
Ovis aries |
| Characteristics |
tissue: Fat Tail
breed: thin tail breed 'Zel'
age: 7 months
|
| Treatment protocol |
No treatment
|
| Growth protocol |
All lambs were weaned at 90 days of age and then fostered under similar conditions at the research station of the Faculty of Agriculture, University of Tabriz, Tabriz, Iran (Khalatpoushan). The lambs were housed in individual pens and provided the same diet ad libitum for 120 days. Animals were slaughtered after 7 months.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent using manufacturer instractions
PolyA enriched RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Fastqc-Trimmomatics-Tophat-Cufflink-Cuffmerge-cuffdiff-rstudio-Enrichment analysis-Network analysis
Sequenced reads were trimmed for adaptor and low-quality sequence
Clean reads mapped to Ovis-aries whole genome using Tophat software
Assembled reads
individual transcripts were merged to form a single transcript assembly
merged transcript was used for quantification of the transcripts
Genome_build: Oar_v3.1
Supplementary_files_format_and_content: Tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Jan 02, 2020 |
| Last update date |
Nov 22, 2021 |
| Contact name |
Sana Farhadi |
| E-mail(s) |
[email protected]
|
| Organization name |
Tabriz University
|
| Street address |
Golgasht street
|
| City |
Tabriz |
| ZIP/Postal code |
5166/15731 |
| Country |
Iran |
| |
|
| Platform ID |
GPL15670 |
| Series (1) |
| GSE142841 |
Signaling pathways, genes and gene networks associated with fat deposition in the tail of different breeds of sheep |
|
| Relations |
| BioSample |
SAMN13708456 |
| SRA |
SRX7494656 |
