|
| Status |
Public on Aug 18, 2009 |
| Title |
WT 60 min rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WT cells 60 minutes after irradiation
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain background: YPH499
genotype: WT
|
| Treatment protocol |
Cells were briefly resuspended in water and irradiated (or mock treated for unirradiated samples) with 100 J/m2 UV-C light with a Stratalinker 1800 (Stratagene). Cells were then resuspended in fresh YPD and allowed to recover for the indicated times by incubation with shaking at 30ºC.
|
| Growth protocol |
Cells were grown overnight in liquid YPD with shaking at 30ºC to an OD600 = 1.0.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a hot acid phenol method followed by sodium acetate/ethanol precipitation.
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis, cRNA synthesis, biotin labelling and fragmentation were performed using Affymetrix GeneChip reagents according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization of labelled, fragmented cRNA to Affymetrix Yeast Genome 2.0 arrays was performed using Affymetrix GeneChip reagents according to the manufacturer's instructions.
|
| Scan protocol |
Scanning was done on the Affymetrix GeneChip System according to the manufacturer's instructions.
|
| Description |
Gene expression data for WT yeast cells 60 minutes after UV irradiation (100 J/m2)
|
| Data processing |
The data was quantile normalized and expression estimates were generated using the Bioconductor GCRMA (version 2.10.0) package in R (version 2.6.2).
|
| |
|
| Submission date |
Jun 24, 2009 |
| Last update date |
Aug 18, 2009 |
| Contact name |
Kunal Poorey |
| E-mail(s) |
[email protected]
|
| Phone |
434-982-3237
|
| Fax |
434-924-5069
|
| Organization name |
University of Virginia Health System
|
| Department |
Biomedical Sciences: Biochemistry, Molecular Biology & Genetics
|
| Lab |
Auble Lab
|
| Street address |
1340 Jefferson Park Avenue
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE16799 |
Expression data for UV time course in Saccharomyces cerevisiae wild-type and mutant strains |
|
