|
| Status |
Public on Dec 10, 2019 |
| Title |
B_BG_MTB |
| Sample type |
SRA |
| |
|
| Source name |
macrophage_BG_24hr_5d_media_MTB_4h
|
| Organism |
Homo sapiens |
| Characteristics |
donor: Donor_B
tissue: Adult Human Peripheral Blood
cell type: macrophage
treatment protocol: BG_24hr_5_day_media_MTB_4hr
|
| Treatment protocol |
Cells were collected in Trizol.
|
| Growth protocol |
Monocytes were negatively selected from peripheral blood and cultured in RPMI and 10% human serum for 6 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNEasy mini kit.
Libraries were prepared by Novogene
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
MMSEQ package was used to infer gene expression levels. Reads were mapped to the genome using Bowtie1
Genome_build: hg19
Supplementary_files_format_and_content: RNA files are in a .gene.mmseq.txt format, containing counts per gene and log RPKM.
|
| |
|
| Submission date |
Dec 09, 2019 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Boris Novakovic |
| E-mail(s) |
[email protected]
|
| Phone |
0434980073
|
| Organization name |
Murdoch Children's Research Institute
|
| Street address |
Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE141656 |
Transcriptomes of human trained macrophages before and after Mtb exposure |
|
| Relations |
| BioSample |
SAMN13512249 |
| SRA |
SRX7290749 |
