|
| Status |
Public on Jun 18, 2009 |
| Title |
Control, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Endothelial cell
agent: ethanol
dose: 0.1%
|
| Treatment protocol |
Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
|
| Growth protocol |
Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from control HUVEC
|
| Data processing |
Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
|
| |
|
| Submission date |
Jun 17, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Carlos Hermenegildo |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Valencia
|
| Street address |
Avda. Blasco Ibanez 15
|
| City |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16683 |
Human endothelial gene expression under estradiol treatment |
|
| Relations |
| Reanalyzed by |
GSE20125 |
| Reanalyzed by |
GSE119087 |
