|
| Status |
Public on Aug 12, 2011 |
| Title |
VMH_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ventromedial hypothalamic nucleus
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: Balb/c
age: 7-8 weeks
tissue: ventromedial hypothalamic nucleus
|
| Treatment protocol |
Slices (0.5-mm thick) of mouse brain were cut on a Mouse Brain Matrix (Neuroscience, Tokyo), frozen, and the specific regions were punched out bilaterally with a microdissecting needle (gauge 0.5 mm) under a stereomicroscope.
|
| Growth protocol |
Balb/c mice (male) purchased 5 weeks postpartum, were adapted under standard 12 h light/dark cycles (LD) for 2 weeks, before samples were obtained under LD or constant darkness (DD) conditions, every 4 h over 1 day, starting at ZT0.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was prepared from the pooled samples for each region taken at all time points using Trizol reagent (GIBCO BRL).
|
| Label |
biotin
|
| Label protocol |
cDNA samples were subjected to the one-cycle target labeling procedure for biotin labeling by in vitro transcription (Affymetrix)
|
| |
|
| Hybridization protocol |
cRNA was subsequently fragmented and hybridized to the GeneChip according to manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Pooled samples collected at every 4 hours over 1 day
|
| Data processing |
The raw data were summarized with the RMA algorithm.
|
| |
|
| Submission date |
Jun 08, 2009 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Hiroki R Ueda |
| E-mail(s) |
[email protected]
|
| Phone |
+81-78-306-3191
|
| URL |
http://brainstars.org/
|
| Organization name |
RIKEN
|
| Department |
Center for Developmental Biology
|
| Lab |
Functional Genomics Unit
|
| Street address |
2-2-3, Minatojima-minamimachi, Chuo-ku
|
| City |
Kobe, Hyogo |
| ZIP/Postal code |
6500047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE16496 |
Expression profile of adult mouse 51 CNS regions |
|
| Relations |
| Reanalyzed by |
GSM3031827 |
