|
| Status |
Public on Oct 24, 2019 |
| Title |
CD127posCD25neg_D20 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Macaca mulatta |
| Characteristics |
cell type: PBMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were cryopreserved, sorted using FACS and captured using 10x genomics single cell platform
10x single cell 3' v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
PBMC were cryopreserved on date indicated. Cells were thawed on December 8, 2017. Cells were enriched for CD4+ using immunomagnetic kit and sorted for cell surface phenotype-CD3+CD4+CD25negCD127pos (D20)
|
| Data processing |
Cellranger 2.1.1 default-parameters
Monocle
Genome_build: Mmul8
Supplementary_files_format_and_content: tab-delim file of genes(rows) and cells (columns)
|
| |
|
| Submission date |
Oct 21, 2019 |
| Last update date |
Oct 24, 2019 |
| Contact name |
Geoff R Hill |
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
CRD
|
| Lab |
Hill Lab
|
| Street address |
1100 Fairview Ave. N.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL21120 |
| Series (1) |
| GSE139200 |
Single cell RNAseq of adoptively transferred Tregs in rhesus macaques |
|
| Relations |
| BioSample |
SAMN13023001 |
| SRA |
SRX6985751 |
