|
| Status |
Public on Nov 18, 2019 |
| Title |
miR192 mimic-tranfected macrophages in the presence of anti-IL-6 Ab #4 |
| Sample type |
RNA |
| |
|
| Source name |
mouse bone marrow derived macrophages #4
|
| Organism |
Mus musculus |
| Characteristics |
transfected rna: miR192 mimic
treated ab: anti-IL-6 Ab
|
| Treatment protocol |
Control or miR192 mimic was transfected into mouse bone marrow-derived macrophages, and 24hrs later those cells were stimulated with R848 for additional 24hrs together with control or anti-IL-6 Ab in vitro.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using RNesy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Gene expression in miR192 mimic-trasnfected ad anti-IL-6 Ab-treated macrophages
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Oct 11, 2019 |
| Last update date |
Nov 19, 2019 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22438 |
| Series (1) |
| GSE138758 |
Investigation of molecular events underlying the miR192-mediated modulation of inflammatory response |
|
