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| Status |
Public on Oct 10, 2022 |
| Title |
POOL NORMAL 2 |
| Sample type |
RNA |
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| Source name |
Lung FFPE tissue
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| Organism |
Homo sapiens |
| Characteristics |
tissue: Histological Normal Lung Tissue
gender: mixed
age: average age of 56
tobacco use: not provided
Stage: n/a
tnm classification: n/a
tumor size: n/a
nodal status: n/a
distant metastasis: n/a
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| Treatment protocol |
Samples obtained from FFPE tissue blocks were needle microdissected using the stereomicroscope Leica EZ4 (Leica Microsystems, Wetzlar, Germany) before RNA extraction, in order to isolate the target tumor or normal cell populations.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation (Thermo Fisher Scientific, Waltham, MA, USA) according to Goswami RS, Atenafu EG, Xuan Y, Waldron L, Reis PP, Sun T, et al. MicroRNA signature obtained from the comparison of aggressive with indolent non-Hodgkin lymphomas: potential prognostic value in mantle-cell lymphoma. J Clin Oncol [Internet]. 2013;31(23):2903–11.
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| Label |
Taqman
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| Label protocol |
n/a
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
Control
microRNA
pool of 3 individuals: one female, two male
Individual ages: 59, 53, 58
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| Data processing |
The software used was ExpressionSuite Software, V 1.1, from Thermo Fisher. We used normal globalization and two genes as control: RNU44-001094 and RNU48-001006.
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| Submission date |
Oct 10, 2019 |
| Last update date |
Oct 10, 2022 |
| Contact name |
Ana Laura Seneda |
| E-mail(s) |
[email protected]
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| Organization name |
São Paulo State University (UNESP), Faculty of Medicine
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| Department |
Surgery and Orthopedics
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| Street address |
Prof. Mário Rubens Guimarães Montenegro, Avenue - UNESP - Campus de Botucatu
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| City |
Botucatu |
| State/province |
São Paulo |
| ZIP/Postal code |
18618687 |
| Country |
Brazil |
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| Platform ID |
GPL18942 |
| Series (1) |
| GSE138708 |
Typical Lung Carcinoids with Metastasis: Potential Role of MicroRNAs in the Regulation of Adaptive Immunity Associated with Disease: a Case Study |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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